Fat cells to the rescue

F
Hedayatpour A, Ragerdi I, Pasbakhsh P, Kafami L, Atlasi N, Pirhajati Mahabadi V, Ghasemi S, Reza M. Promotion of remyelination by adipose mesenchymal stem cell transplantation in a cuprizone model of multiple sclerosis. Cell J. 2013 Summer;15(2):142-51. Epub 2013 Jul 2.OBJECTIVE:Multiple sclerosis (MS) is an immune-mediated demyelinating disease of the central nervous system (CNS). Stem cell transplantation is a new therapeutic approach for demyelinating diseases such as MS which may promote remyelination. In this study, we evaluate the remyelinating potential of adipose mesenchymal stem cells (ADSCs) and their effect on neural cell composition in the corpus callosum in an experimental model of MS.
MATERIALS AND METHODS: 
This experimental study used adult male C57BL/6 mice. Cultured ADSCs were confirmed to be CD73(+),CD90(+), CD31(-),CD45(-), and labeled by PKH26. Animals were fed with 0.2% w/w cuprizone added to ground breeder chow ad libitum for six weeks. At day 0 after cuprizone removal, mice were randomly divided into two groups: the ADSCs-transplanted group and the control vehicle group (received medium alone). Some mice of the same age were fed with their normal diet to serve as healthy control group. Homing of ADSCs in demyelinated lesions was examined by fluorescent microscope. At ten days after transplantation, the mice were euthanized and their cells analyzed by luxol fast blue staining (LFB), transmission electron microscopy and flow cytometry. 
RESULTS:According to fluorescent cell labeling, transplanted ADSCs appeared to survive and exhibited homing specificity. LFB staining and transmission electron microscope evaluation revealed enhanced remyelination in the transplanted group compared to the control vehicle group. Flow cytometry analysis showed an increase in Olig2 and O4 cells and a decrease in GFAP and Iba-1 cells in the transplanted group.
CONCLUSION: Our results indicate that ADSCs may provide a feasible, practical way for remyelination in diseases such as MS.


Another type of stem cell to remyelinate, but is luxol fast blue sensitive enough to really spot remyelination?

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MouseDoctor

3 comments

  • Is the cuprizone model of demyelination really reflective of the inflammatory-mediated demyelination in MS? I tend to doubt it.

    • What model should be used for remyelination studies?
      EAE, TMVV, etc. they all seem to have their short comings. Or are remyelination studies just putting the cart before the horse……should research focus on the axon?

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