Myelination of the central nervous system is performed by oligodendrocytes, which have been implicated in brain disorders such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an oligodendrocyte reference proteome database. Approximately 11,600 unique peptides were identified and, after stringent filtering, resulted in 2,290 proteins representing 9 distinct biological processes and various molecular classes and functions. Oligodendrocyte-specific proteins such as MBP and CNP as well as proteins involved in multiple sclerosis and schizophrenia were also identified. Proteins of this dataset have also been classified according to their chromosomal origin for providing useful data to the chromosome-centric human proteome project (C-HPP). Given the importance of oligodendrocytes in the aetiology of demyelinating and oligodendrogial disorders, the MO3.13 proteome database will provide a valuable resource. The mass spectrometry proteomics data have been deposited to the ProteomeXchange with identifier PXD000263.
Iwata K, Café-Mendes CC, Schmitt A, Steiner J, Manabe T, Matsuzaki H, Falkai P, Turck CW, Martins-de-Souza D. The human oligodendrocyte proteome. Proteomics. 2013 Oct 24. doi: 10.1002/pmic.201300201. [Epub ahead of print]
When you have an idea what Health looks like you can work out what is different in disease and it may give you ideas of what processes are occurring. This study aims to help understand what the background should be.