CD8 Cells after Immunological Stem Cell Reconstitutions

Arruda LC, de Azevedo JT, de Oliveira GL, Scortegagna GT, Rodrigues ES, Palma PV, Brum DG, Guerreiro CT, Marques VD, Barreira AA, Covas DT, Simões BP, Voltarelli JC, Oliveira MC, Malmegrim KC.Immunological correlates of favourable long-term clinical outcome in multiple sclerosis patients after autologous haematopoietic stem cell transplantation. Clin Immunol. 2016 pii: S1521-6616(16)30098-5.

High dose immunosuppression followed by autologous haematopoietic stem cell transplantation (AHSCT) induces prolonged clinical remission inmultiple sclerosis (MS) patients. However, how patient immune profiles are associated with clinical outcomes has not yet been completely elucidated. In this study, 37 MS patients were assessed for neurological outcomes, thymic function and long-term immune reconstitution after AHSCT. Patients were followed for a mean (SD) of 68.5 (13.9) months post-transplantation and were retrospectively clustered into progression- and non-progression groups, based on Expanded Disease Status Scale (EDSS) outcomes at last visit. After AHSCT, both patient groups presented increased regulatory T-cell subset counts, early expansion of central- and effector-memory CD8+T-cells and late thymic reactivation. However, the non-progression group presented early expansion of PD-1-expressing CD8+T-cells. Here, we suggest that along with increased numbers of regulatory T-cell subsets, PD-1 inhibitory signaling is one possible immunoregulatory mechanism by which AHSCT restores immune tolerance in MS patients.

What drives MS, what controls MS?. One thing that is increasingly clear is that myoablative haematopeoitic stem cell therapy is very effective at controlling relapsing MS and it is clear that relapsing MS is a product of the immune system enetering the CNS. 

This week, I saw the data from the HALT-MS study presented (BEAM myoablation  followed by HSCT) and is high efficacy, and the way the immune system reconstituted was reported to. The authors said they would do a “guest post” on this.

However this is another study looking at immune reconstitution after the transplant. The first wave of reconstitution is the innate system and the neutrophil which has to get you some protection from infection, but which lmymphocytes appear and how does this relate to disease activity. With Alemtuzmab long-term depletion of CD4 and CD8 depletion and transient CD19 B cell depletion is associated with a good outcome for MS, not secondary B cell autoimmunities:-( 

In this study CD8 and T regulatory subsets rapidly appear, is this due to regulation or in response to viruses that lurk in our environments?, However there was a population of CD8 cells enriched in those that didnot reactivate their disease and the expressed PD-1. Programmed cell death protein 1, also known as CD279 is a cell surface receptor that binds two molecules, PD-L1 and PD-L2.

PD-1, plays an important role in down regulating the immune system by preventing the activation of T-cells, which in turn reduces autoimmuity and promotes self-tolerance. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen antigen specific T-cells, while simultaneously reducing death in regulatory T cells. 

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  • Is there any data on Ocrelizumabe and Cladrbine acting on the depletion of CD4 cells and CD8?
    I was quite confused about the positive results of Alemtuzumab being by just act in these cells, and not in secondary autoimmunity B.

    Prof. Michael Pender has some studies showing a defect in CD8 + effector cells um people with MS and that may just be caused by the action of the Epstein Barr Virus…

    • There is data. I believe the depletion of T cells is minor (5-10%) for ocrelizumab and I think and for cladribine there is about a 20% CD8 T cell depletion compared to about +90% for alemtuzumab, so there cells available to deal with PML. CD4 depletion is modest at about 50% depending on dose for CLAD compared to alemtuzumab but it hits memory cells.

      I think the issue is that alemtuzumab does not deplete B cells for a long time. For cladribine depletion is marked and profund and with ocrelizumab you get a depletion every six months. However with alemtuzumab you get transient depletion and a not only recovery but a B cell overshoot that occurs at a time when there is a pausity of CD4 and CD8 T cell regulation. For ocrelizumab and CLAD B cells return in the presence of regulation and so no autoimmunities…Just a hypothesis.

      This data is out there but it is amazing that the companires have not published this data in proper publications. It is amazing that when you look at the CARE I & CARE II publications the effects on T and B cells were mentioned but data was not shown in the original papers. There was some text but only figures of CAREII data showed lymphocyte depletion was shown showing a depletion at each injection. However two becomes one
      the T cells are depleted out of sight whilst the B cells proliferate like crazy so you have excessive levels. Mix the two and you miss this. Why is this the reason why there are B cell autoimmunities. Likewise they have failed to present the data on alemtuzumab reactive antibodies in a publication, dribbling the data out in abstracts published years ago.
      The level of anti alemtuzumab antibodies is amazingly high (>90%), perhaps not surprsing there are infusion reactions

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