Mondal S, Rangasamy SB, Ghosh S, Watson RL, Pahan K.
Neurochem Res. 2017 Mar 7. doi: 10.1007/s11064-017-2214-z. [Epub ahead of print]
Developing a new and effective therapeutic approach against multiple sclerosis (MS) is always an important area of research. RNS60 is a bioactive aqueous solution generated by subjecting normal saline to Taylor-Couette-Poiseuille flow under elevated oxygen pressure. Recently we have demonstrated that RNS60, administered through intraperitoneal injection, ameliorated clinical symptoms and disease progression of experimental allergic encephalomyelitis (EAE), an animal model of MS. Since the intravenous route is not preferred for treating a chronic condition, we tested if nebulization of RNS60 could attenuate the disease process of adoptively-transferred EAE in mice. Although we could not directly image RNS60 after nebulization, nebulized Alexa680 reached spleen, spinal cord and different parts of the brain. Nebulization of RNS60 starting from the acute phase attenuated clinical symptoms of relapsing-remitting EAE in female SJL/J mice. RNS60 nebulization also inhibited perivascular cuffing, maintained the integrity of blood-brain and blood-spinal cord barriers, suppressed inflammation, normalized the expression of myelin genes, and blocked demyelination in the CNS of EAE mice. On the immunomodulatory front, nebulization of RNS60 to EAE mice led to the enrichment of anti-autoimmune regulatory T cells (Tregs) and suppression of autoimmune Th17 cells. Together, these results suggest that nebulization of RNS60 may be used to control aberrant immune responses in MS and other autoimmune disorders.
We showed many years ago that if you give too large a volume of salt solution you could inhibit EAE as well or better than many commercial drugs.
It was the building site effect..the animals were being stressed by the osmotic imbalance causing immunosuppression, just like noise stresses the animals and stops them getting disease (the building site effect).
We have the Yale group telling us that saline gives us autoimmunity, yet this current study goes the other way and says that a modified salt solution does the trick to inhibit disease. The use taylor coutte Poiseuilleois flow method with oxygen pressure and made it into an aerosol and it largely stopped EAE and it inhibited Th17 and T reg cells..Salt here we come.
How’s it inhibit them T regs/Th17?